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recombinant full length human chromogranin  (Creative BioMart)


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    Creative BioMart recombinant full length human chromogranin
    Recombinant Full Length Human Chromogranin, supplied by Creative BioMart, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+full+length+human+chromogranin+a/chromogranin++cga/us11643660-257-204-198
    Average 90 stars, based on 1 article reviews
    recombinant full length human chromogranin - by Bioz Stars, 2026-10
    90/100 stars

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    Recombinant:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. LDH5: Lactate Dehydrogenase 5, Human (manufactured by Meridian Life Science, Inc., Catalogue number: # A38558H-100) CgA: Recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalogue number: # CHGA 26904TH) IL-6: IL-6, manufactured by Human, Recombinant (manufactured by PeproTech, Catalogue number: #200-06) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) The results of measuring the binding between the respective types of the LDH5-binding nucleic acid molecule and LDH5 are shown in FIG. 15, the results of measuring the binding between the respective types of the CgA-binding nucleic acid molecules to CgA are shown in FIGS. 16 and 17, and the results of measuring the binding between the respective types of IL-6-binding nucleic acid molecules and IL-6 are shown in FIG. 18. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. LDH5: Lactate Dehydrogenase 5, Human (manufactured by Meridian Life Science, Inc., Catalogue number: # A38558H-100) CgA: Recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalogue number: # CHGA 26904TH) IL-6: IL-6, manufactured by Human, Recombinant (manufactured by PeproTech, Catalogue number: #200-06) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) The results of measuring the binding between the respective types of the LDH5-binding nucleic acid molecule and LDH5 are shown in FIG. 12, and the results of measuring the binding between the respective types of IL-6-binding nucleic acid molecules and IL-6 are shown in FIG. 13. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as control 1 and control 2, examination of the binding was performed in the same manner except that, in control 1, bovine serum albumin (BSA) was used as the following analyte, and in control 2, chromogranin A (CgA, manufactured by Creative BioMart) was used as the following analyte in a system for examining the binding of the α-amylase-binding nucleic acid molecules 1 and the α-amylase was used as the following analyte in a system for examining the binding of the sIgA-binding nucleic acid molecules. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (manufactured by BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dA) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(T) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte flow rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: # CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: # A7906) The results of measuring the binding between the α-amylase-binding nucleic acid molecule 1 and the α-amylase are shown in FIG. 1, and the results of measuring the binding between the sIgA-binding nucleic acid molecule and sIgA are shown in FIG. 2. ..

    Binding Assay:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. LDH5: Lactate Dehydrogenase 5, Human (manufactured by Meridian Life Science, Inc., Catalogue number: # A38558H-100) CgA: Recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalogue number: # CHGA 26904TH) IL-6: IL-6, manufactured by Human, Recombinant (manufactured by PeproTech, Catalogue number: #200-06) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) The results of measuring the binding between the respective types of the LDH5-binding nucleic acid molecule and LDH5 are shown in FIG. 15, the results of measuring the binding between the respective types of the CgA-binding nucleic acid molecules to CgA are shown in FIGS. 16 and 17, and the results of measuring the binding between the respective types of IL-6-binding nucleic acid molecules and IL-6 are shown in FIG. 18. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. LDH5: Lactate Dehydrogenase 5, Human (manufactured by Meridian Life Science, Inc., Catalogue number: # A38558H-100) CgA: Recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalogue number: # CHGA 26904TH) IL-6: IL-6, manufactured by Human, Recombinant (manufactured by PeproTech, Catalogue number: #200-06) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) The results of measuring the binding between the respective types of the LDH5-binding nucleic acid molecule and LDH5 are shown in FIG. 12, and the results of measuring the binding between the respective types of IL-6-binding nucleic acid molecules and IL-6 are shown in FIG. 13. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as control 1 and control 2, examination of the binding was performed in the same manner except that, in control 1, bovine serum albumin (BSA) was used as the following analyte, and in control 2, chromogranin A (CgA, manufactured by Creative BioMart) was used as the following analyte in a system for examining the binding of the α-amylase-binding nucleic acid molecules 1 and the α-amylase was used as the following analyte in a system for examining the binding of the sIgA-binding nucleic acid molecules. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (manufactured by BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dA) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(T) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte flow rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: # CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: # A7906) The results of measuring the binding between the α-amylase-binding nucleic acid molecule 1 and the α-amylase are shown in FIG. 1, and the results of measuring the binding between the sIgA-binding nucleic acid molecule and sIgA are shown in FIG. 2. ..

    Control:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as control 1 and control 2, examination of the binding was performed in the same manner except that, in control 1, bovine serum albumin (BSA) was used as the following analyte, and in control 2, chromogranin A (CgA, manufactured by Creative BioMart) was used as the following analyte in a system for examining the binding of the α-amylase-binding nucleic acid molecules 1 and the α-amylase was used as the following analyte in a system for examining the binding of the sIgA-binding nucleic acid molecules. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (manufactured by BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dA) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(T) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte flow rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: # CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: # A7906) The results of measuring the binding between the α-amylase-binding nucleic acid molecule 1 and the α-amylase are shown in FIG. 1, and the results of measuring the binding between the sIgA-binding nucleic acid molecule and sIgA are shown in FIG. 2. ..

    SPR Assay:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as control 1 and control 2, examination of the binding was performed in the same manner except that, in control 1, bovine serum albumin (BSA) was used as the following analyte, and in control 2, chromogranin A (CgA, manufactured by Creative BioMart) was used as the following analyte in a system for examining the binding of the α-amylase-binding nucleic acid molecules 1 and the α-amylase was used as the following analyte in a system for examining the binding of the sIgA-binding nucleic acid molecules. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (manufactured by BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dA) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(T) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte flow rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: # CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: # A7906) The results of measuring the binding between the α-amylase-binding nucleic acid molecule 1 and the α-amylase are shown in FIG. 1, and the results of measuring the binding between the sIgA-binding nucleic acid molecule and sIgA are shown in FIG. 2. ..

    Modification:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as control 1 and control 2, examination of the binding was performed in the same manner except that, in control 1, bovine serum albumin (BSA) was used as the following analyte, and in control 2, chromogranin A (CgA, manufactured by Creative BioMart) was used as the following analyte in a system for examining the binding of the α-amylase-binding nucleic acid molecules 1 and the α-amylase was used as the following analyte in a system for examining the binding of the sIgA-binding nucleic acid molecules. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (manufactured by BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dA) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(T) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte flow rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: # CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: # A7906) The results of measuring the binding between the α-amylase-binding nucleic acid molecule 1 and the α-amylase are shown in FIG. 1, and the results of measuring the binding between the sIgA-binding nucleic acid molecule and sIgA are shown in FIG. 2. ..

    Injection:

    Article Title: Nucleoside derivative or salt thereof, polynucleotide synthesis reagent, method for producing polynucleotide, polynucleotide, and method for producing binding nucleic acid molecule
    Article Snippet: .. Further, as a control, examination of the binding was performed in the same manner except that, in a system examining the binding of the amylase-binding nucleic acid molecule, the sIgA, chromogranin A (CgA, Creative BioMart), and bovine serum albumin (BSA) were used as the following analytes, and in a system examining the binding of the sIgA-binding nucleic acid molecule, the amylase, CgA, and BSA were used as the following analytes. (SPR Measurement Conditions) Measurement device: ProteOnTM XPR36 (BioRad) Measurement chip: ProteOnTM NLC Sensor Chip (manufactured by BioRad) Ligand 1: poly(dT) (20-mer) with the 5′ end thereof being modified with biotin: 5 μmol/L Buffer: 40 mmol/L HEPES, 125 mmol/L NaCl, 1 mmol/L MgCl2, 5 mmol/L KCl, 0.01% Tween® 20, pH 7.4 Ligand 2: buffer containing a binding nucleic acid molecule with poly(A) (20-mer) added to the 3′ end at 200 nmol/L Ligand flow rate: 25 μL/min, 80 sec Analyte: buffer containing a target at 400 nmol/L Analyte Flow Rate: 50 μL/min Contact Time: 120 sec Dissociation: 300 sec Amylase: α-amylase (manufactured by Lee Biosolutions, Catalogue number: #120-10) sIgA: IgA (Secretory), Human (manufactured by MP Biomedicals, LLC-Cappel Products, Catalogue number: #55905) CgA: recombinant full length Human Chromogranin A (manufactured by Creative BioMart, Catalog number: #CHGA 26904TH) BSA: Bovine Serum Albumin (manufactured by SIGMA, Catalogue number: #A7906) After injection of the ligand 2, signal intensity (RU) measurement was performed. ..



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